Journal: Neuroscience

Article Title: Chronic intermittent hypoxia induces robust astrogliosis in an Alzheimer’s disease-relevant mouse model

doi: 10.1016/j.neuroscience.2018.11.040

Figure Lengend Snippet: (A) Example photomicrographs of 6E10 immunohistochemical staining in the cortex in 11-12 month old APP/PS1 KI (KI) mice following 28 days of chronic intermittent hypoxia exposure (IH). (B) Example of the computergenerated markup created by the positive pixel algorithm demonstrating the ability of the algorithm to accurately detect the immunostaining. Colors, yellow < orange < red, indicate staining intensities low < medium < high, respectively. (C) Quantification of the 6E10+ staining found that IH exposure did not increase histological amyloid plaque load in the neocortex, corpus callosum, subiculum, CA1/2, dentate gyrus, and thalamus. Quantitative Aβ ELISA of PBS soluble and FA soluble found no effect of IH exposure on Aβ40 (D) or Aβ42 in the neocortex (E). Each circle represents an individual animal.

Article Snippet: Primary antibodies used included mouse biotin anti-β-Amyloid, 1-16 antibody (6E10 Aβ) (Covance cat#39340-200; (1:3000) Biolegend, San Diego, CA) and rabbit anti-glial fibrillary acid protein (GFAP) (Dako Cat#Z0334; (1:10000); Dako, Glostrup, Denmark).

Techniques: Immunohistochemical staining, Staining, Immunostaining, Enzyme-linked Immunosorbent Assay